Ex Vivo Testing
Design and develop appropriate assays using whole lens or lens epithelial explant-based ex vivo system to test the toxicity/activity of the drug/inhibitor/polymer
Techniques
Fibrosis explant-based/whole lens-based assay to assess the anti-fibrotic potential of the drug.
Cytotoxicity analyses (Annexin V, MTT etc.)
DNA/RNA-based genetic assays (RT-PCR, PCR array, NanoString etc.)
Western blot and Immunofluorescence-based assays to track the downstream signalling